Journal: Journal for Immunotherapy of Cancer

Article Title: Redirecting cytomegalovirus immunity against pancreas cancer for immunotherapy

doi: 10.1136/jitc-2025-012969

Figure Lengend Snippet: MCMV T cells are highly activated and cytotoxic within tumors. Immunophenotyping and cytokine production analysis of the TILs described in (25 days post-tumor implantation). (n=5/group). (A) Representative histograms of m45-specific CD8 T cells isolated from tumors (top) and MFI quantification (bottom). (B) Tumors were subjected to CD45+TIL magnetic separation and the purified cells were incubated with the six MCMV peptides for 4 hours, in the presence of GolgiPlug. The graphs show IFN-γ and TNF production in CD8 or CD4 Tconv cells. Results were compared by one-way ANOVA with Tukey correction. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. ANOVA, analysis of variance; Gzm A and B, granzymes A and B; IFN, interferon; LAG3, lymphocyte activation gene 3 ; MCMV, murine cytomegalovirus; MFI, mean fluorescence intensity; Prf, perforin; PD-1, programmed cell death protein-1; Tconv, conventional T cell; TILs, tumor-infiltrating lymphocytes; TIM3, T cell immunoglobulin and mucin domain 3; TNF, tumor necrosis factor.

Article Snippet: When indicated, mice were intraperitoneally injected with anti-programmed cell death protein-1 (PD-1) (10 mg/kg two times a week, Bio X Cell, 29F.1A12), anti-Interleukin 10 Receptor (IL10R) (200 μg, once a week, Bio X Cell, 1B1.3A), anti-CD8 (200 μg, two times a week, Bio X Cell, YTS 169.4), anti-CD4 (100 μg two times a week, Bio X Cell, GK1.5) antibodies, or gemcitabine (5 mg/kg, two times a week, Selleck Chem).

Techniques: Tumor Implantation, Isolation, Purification, Incubation, Activation Assay, Fluorescence